Fatty Acid Oxidation (FAO) Assay Kit                                                                                                                                                              Price

Product Description: Fatty acids provide more ATP per carbon than carbohydrates such as glucose. In tissues with high energy requirement,
such as heart, up to 50 – 70% of energy comes from fatty acid beta-oxidation, which converts fatty acids to acetyl-CoA with concomitant production
of FADH
2 and NADH in the mitochondria. This strictly aerobic oxidative pathway consists of four distinct steps: acyl-CoA dehydrogenation by acyl-
CoA dehydrogenase, hydration by enoyl-CoA hydratase, dehydrogenation by 3-hydroxyacyl-CoA dehydrogenase, and thiolytic cleavage by beta-
ketothiolase. The importance of the beta-oxidation system in humans is exemplified by the existence of a group of genetic diseases categorized as
fatty acid oxidation disorders (FAODs), which are a heterogeneous group of defects in fatty acid transport and oxidation, and are inherited as
autosomal recessive disorders, having a wide range of clinical presentations. In addition, evidence indicates that regulation of activity of FAO during
fetal development is not only important for fetal life but also has implications for health and disease in adulthood. The measurement of FAO activity
thus provides valuable insights into the pathophysiologic and metabolic basis of many diseases. The non-radioactive FAO assay is based on
oxidation of the substrate octanoyl-CoA. Generation of NADH is coupled to the reduction of the tetrazolium salt INT to formazan. The intensity of the
red-colored formazan is proportional to increased FAO activity. The assay solution and substrate should be stored in aliquots at -80ºC.

Kit Components:

FAO Assay Solution: 5 ml, store at -70°C (for 100 wells)
20x Octanoyl-CoA: 0.25 ml, store at -70ºC                                 
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Tris

Related kits: HK Enzyme Assay, G6PD Enzyme Assay, Adenylate Kinase Assay

Citation:
Kwong et al
Metabolic role of fatty acid binding protein 7 in mediating triple-negative breast cancer cell death via PPAR-alpha signaling
J. Lipid Res. 60:1807, 2019

Cheng et al
Targeting DGAT1 ameliorates glioblastoma by increasing fat catabolism and oxidative stress.
Cell Metabolism 32, 229-242, 2020
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Tissue homogenates were
prepared from normal hamster
heart (F1B strain) and failing
heart (TO2 strain). FAO activities
were assayed by the FAO Assay
kit, showing that the failing
hamster heart exhibits reduced
capacity for fatty acid oxidation.