Fatty Acid Oxidation (FAO) Assay Kit (medium chain or long chain fatty acid)                                                                                                Price

Product Description: Fatty acids provide more ATP per carbon than carbohydrates such as glucose. In tissues with high energy requirement,
such as heart, up to 50 – 70% of energy comes from fatty acid beta-oxidation, which converts fatty acids to acetyl-CoA with concomitant production
of FADH
2 and NADH in the mitochondria. This strictly aerobic oxidative pathway consists of four distinct steps: acyl-CoA dehydrogenation by acyl-
CoA dehydrogenase, hydration by enoyl-CoA hydratase, dehydrogenation by 3-hydroxyacyl-CoA dehydrogenase, and thiolytic cleavage by beta-
ketothiolase. The importance of the beta-oxidation system in humans is exemplified by the existence of a group of genetic diseases categorized as
fatty acid oxidation disorders (FAODs), which are a heterogeneous group of defects in fatty acid transport and oxidation, and are inherited as
autosomal recessive disorders, having a wide range of clinical presentations. In addition, evidence indicates that regulation of activity of FAO during
fetal development is not only important for fetal life but also has implications for health and disease in adulthood. The measurement of FAO activity
thus provides valuable insights into the pathophysiologic and metabolic basis of many diseases. The non-radioactive FAO assay is based on
oxidation of the substrate octanoyl-CoA (for medium chain fatty acid) or palmitoyl-CoA (for long chain fatty acid). Generation of NADH is coupled to
the reduction of the tetrazolium salt INT to formazan. The intensity of the red-colored formazan is proportional to increased FAO activity. The assay
solution and substrate should be stored in aliquots at -80ºC.

Kit Components:

FAO Assay Solution: 5 ml, store at -80°C (for 100 wells)
20x Octanoyl-CoA (#E-141M) or Palmitoyl-CoA (#E-141L): 0.25 ml, store at -80ºC                                 
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: TX-100, DMSO, INT, acetic acid, Tris

Related kits: HK Enzyme Assay, G6PD Enzyme Assay, Adenylate Kinase Assay
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Mice were fed methionine-choline
deficient (MCD) diet for 14 weeks.
Heart tissues were homogenized
and FAO enzyme activities were
assayed by the FAO Assay kit.