Fatty Acid Oxidation (FAO) Assay Kit Price
Product Description: Fatty acids provide more ATP per carbon than carbohydrates such as glucose. In tissues with high energy requirement,
such as heart, up to 50 – 70% of energy comes from fatty acid beta-oxidation, which converts fatty acids to acetyl-CoA with concomitant production
of FADH2 and NADH in the mitochondria. This strictly aerobic oxidative pathway consists of four distinct steps: acyl-CoA dehydrogenation by acyl-
CoA dehydrogenase, hydration by enoyl-CoA hydratase, dehydrogenation by 3-hydroxyacyl-CoA dehydrogenase, and thiolytic cleavage by beta-
ketothiolase. The importance of the beta-oxidation system in humans is exemplified by the existence of a group of genetic diseases categorized as
fatty acid oxidation disorders (FAODs), which are a heterogeneous group of defects in fatty acid transport and oxidation, and are inherited as
autosomal recessive disorders, having a wide range of clinical presentations. In addition, evidence indicates that regulation of activity of FAO during
fetal development is not only important for fetal life but also has implications for health and disease in adulthood. The measurement of FAO activity
thus provides valuable insights into the pathophysiologic and metabolic basis of many diseases. The non-radioactive FAO assay is based on the
oxidation of octanoyl-CoA and generation of NADH, which is coupled to the reduction of the tetrazolium salt INT to formazan. The intensity of the red-
colored formazan is proportional to increased FAO activity. The assay solution and substrate should be stored in aliquots at -80ºC.
FAO Assay Solution: 5 ml, store at -80°C (for 100 wells)
20x FAO Substrate: 0.25 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC
MSDS: TX-100, DMSO, INT, acetic acid, Hepes
Related kits: HK Enzyme Assay, G6PD Enzyme Assay, Adenylate Kinase Assay
Biomedical Research Service
& Clinical Application
Mice were fed methionine-choline
deficient (MCD) diet for 14 weeks.
Heart tissues were homogenized
and FAO enzyme activities were
assayed by the FAO Assay kit.