E coli Competent Cells                                                                                                                                                                                    Price

Product Description: Bacteria can take up and stably maintain exogenous plasmid DNA in a process referred to as genetic transformation. The
efficiency of transformation depends on a transitory state of host cell competence, which, although varies significantly among different bacterial
strains, can be greatly enhanced through chemical treatments or electroporation. For most cloning experiments, however, competent cells
capable of generating 10^6 - 10^7 cfu/ug plasmid DNA can be routinely used. Some but not all E. coli strains can be made highly competent using
an enhanced calcium chloride-based method. A transformation efficiency of up to 10^8 cfu/ug pBluescript plasmid DNA can be obtained with our
DH5alpha competent cells. The competent cells, if stored at -80ºC in aliquots, can remain competent indefinitely.

Contents:

E. coli DH5alpha competent cells: 30 x 50 ul, store at -80°C

Related kits: Plasmid DNA Isolation, High-Range DNA Markers, Low-Range DNA Markers

Citation:
Lin et al
Adenoviral expression of vascular endothelial growth factor splice variants differentially regulate bone marrow-derived mesenchymal stem cells.
J Cell Physiology 216:458-68, 2008
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E. coli DH5alpha competent cells were transformed with
p
Bluescript plasmid DNA.
Biomedical Research Service
& Clinical Application
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