NANO DNA Transfection Kit                                                                                                                                                                           Price

PRODUCT DESCRIPTION: The most frequently used DNA transfer methods are those based on the use of calcium phosphate, DEAE dextran,
liposome, and electroporation (BioTechniques 13:598,1992). However, efficiency of DNA transfection and reagent toxicity vary widely between
different cell types and cell lines. Our
NANO DNA Transfection Kit is based on incorporation of DNA into uniquely formulated nanoparticles in the
presence of a polymer. The nanoparticle formulation allows researchers to obtain high DNA transfection efficiency (see figure below for
comparison of different DNA transfection reagents using HepG2 cells)
. Each cell type should be optimized by researchers to assess potential
reagent toxicity and transfection efficiency.
The kit is stable for at least one year if handled and stored properly.

Kit Components:

NANO Solution A: 3 ml, store at 4°C (sufficient for 100 wells of a 24-well plate)
NANO Solution B: 3 ml, store at 4°C
NANO Solution C: 30 ml, store at 4°C

Citation:
Lin et al.
sFRP2 activates Wnt/beta-catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix
remodeling.
Am J. Physiol. 311:C710-C719, 2016
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Mesenchymal stem cells were transfected
using
NANO DNA Transfection Kit. Red
colors depict the intracellular locations of
the EMA-conjugated DNA.
Alphabetical Listing
HepG2 cells were transfected with a luciferase reporter
plasmid DNA
using NANO DNA Transfection Kit and three
other transfection reagents for comparison.
Cells were
maintained in DMEM containing 10% FBS for 3 days after
transfection. Luciferase assay was performed using our
Luciferase Assay Kit (#E-126). The result shows that our
NANO DNA Transfection Kit is far more superior to other DNA
transfection reagents.