DNA Transfection Kit                                                                                                                                                                                        Price

PRODUCT DESCRIPTION: The most frequently used DNA transfer methods are those based on the use of calcium phosphate, DEAE dextran,
liposome, and electroporation (BioTechniques 13:598,1992). However, efficiency of DNA transfection and reagent toxicity vary widely between
different cell types and cell lines. Our DNA Transfection Kit is based on incorporation of DNA into uniquely formulated nanoparticles in the
absence (Solution A) or presence (Solution B) of a polymer. The two nanoparticle formulations allow you to optimize transfection efficiency for
different cell systems. The use of Solution A or Solution B should be determined empirically for each cell type. For instance, transfection of
C2C12 myocytes with Solution A can yield a 10-fold higher transfection efficiency than with Solution B. On the other hand, Sol8 myocytes can be
more efficiently transfected with Solution B. The kit is stable for at least one year if handled and stored properly.

Kit Components:

DNA solution: 10 ml, store at 4°C
Hepes solution: 0.2 ml, store at 4°C
Solution A: 10 ml, store at 4°C
Solution B: 10 ml, store at 4°C

MSDS: DEAE dextran, Hepes

Related kits: Genomic DNA Isolation, Whole Blood DNA Isolation, Gel Shift Assay, Maxi-Plasmid DNA Purification, E coli Competent Cell
Preparation Kit

Citation:
Lin et al.
sFRP2 activates Wnt/beta-catenin signaling in cardiac fibroblasts: differential roles in cell growth, energy metabolism, and extracellular matrix
remodeling.
Am J. Physiol. 311:C710-C719, 2016
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Biomedical Research Service
& Clinical Application
Mesenchymal stem cells were
transfected using the DNA Transfection
Kit. Red colors depict the intracellular
locations of the EMA-conjugated DNA.
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