Catalase Assay Kit                                                                                                                                                                                            Price

Product Description: Catalase, present in nearly all aerobic cells, serves to protect the cell against oxidative damage caused by hydrogen
peroxide (H2O2). Hydrogen peroxide, although not a free radical, is highly reactive. Catalase catalyzes its decomposition into molecular oxygen
and water without the production of free radicals. Catalase also uses hydrogen peroxide to oxidize toxins including phenols, formic acid,
formaldehyde, and alcohols. The beef liver catalase monomer consists of 506 amino acids, a heme prosthetic group, and one NADH molecule.
The enzyme is among the most efficient known, with rates approaching 200,000 catalytic events/second/subunit, which is near the
diffusion-controlled limit. Our catalase assay system is based on the ability of hydrogen peroxide to oxidize an azo chromogen with an
accompanying increase in O.D.410-420 nm, which can be conveniently quantified by spectroscopy. The presence of catalase reduces the amount
of hydrogen peroxide, thus inhibiting the oxidation of the azo chromogen and the appearance of the yellowish color. This inhibition of chromogen
oxidation is thus proportional to the activity of catalase present in the sample. As little as 103-104 cells can be used per assay. The assay is
simple, non-radioactive, and very sensitive. Each kit is sufficient for 250 microassays. The assay requires a 50ºC incubator.

Kit Components:

Catalase Substrate Solution: 25 ml, store at 4ºC
Catalase Assay Solution: 25 ml, store at 4ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: H2O2, TX-100

Related kits: Lipid Peroxidation Assay Kit, Peroxide Assay Kit

Citation:
Chiu et al
PARP mediates structural alterations in diabetic cardiomyopathy
J Molecular Cellular Cardiology 45:385-393, 2008

Burkart et al
Insulin resistance in human iPS cells reduces mitochondrial size and function
Scientific Reports 6:22788, 2016

PLoS One, 5:e12427, 2010

Cancer Res, 69: 5560-5567, 2009

AGE 36:21, 2014
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Biomedical Research Service
& Clinical Application
Muscle tissue extracts were prepared following
mesenchymal stem cell (MSC) therapy for
hamster muscular dystrophy (MD). The stem
cell-injected muscle (MD/MSC) displays a
normalized level of catalase activity.
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