Caspase 3 Assay Kit                                                                                                                                                                                        Price

Product Description: Members of the caspase family of cysteine proteases are critically involved in coordinating the events occurring during
apoptosis. Caspases are activated in response to diverse death signals and ultimately demolish the cell through limited proteolysis of more than
400 protein substrates. Caspases are initially produced as inactive monomeric procaspases that require dimerization and often cleavage for
activation. In humans, caspase-8, caspase-9, and caspase-10 play upstream roles, i.e., initiator caspases, by coupling cell death to the
downstream effector or executioner caspases (caspase-3, caspase-6, and caspase-7). Various apoptotic pathways exist (extrinsic vs. intrinsic)
that can be distinguished by the adapter proteins and initiator caspases involved. While the initiator caspases primarily cleave their own
precursors and the downstream effector caspases, the bulk of the proteolysis that takes place during apoptosis is carried out by the executioner
caspases. The Caspase 3 activity assay kit is based on proteolytic hydrolysis of the chromogenic peptide substrate
Asp-Met-Gln-Asp-p-nitroanilide. Cleavage of pNA from the substrate increases absorbance at 405 nm (extinction coefficient= 9.5 mM-1cm-1),
allowing for sensitive and quantitative assay of Caspase 3 activity present in tissue/cell lysates. Kit components are stable for at least 1 year if
stored and handled properly.  

Kit Components:

10x Caspase 3 Buffer: 0.6 ml, store at 4°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
50x Caspase 3 Substrate: 0.1 ml, store at -20°C (for 100 assays)
100x DTT: 0.1 ml, store at -20°C

MSDS: Tris, DTT, NP40, pNA, DMSO, Caspase 3 substrate

Related products: AAP Assay, CTSB Assay, CTSC Assay, DPP-4 Assay
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Apoptosis of cultured HepG2
hepatocytes was induced by
overexpression of tissue inhibitor
of matrix metalloproteinase-3
(TIMP-3). Caspase 3 activities were
determined using cell lysates.