Cathepsin C (CTSC or DPP-1) Assay Kit Price
Product Description: Cathepsin C (CTSC or DPP-1), an abundant lysosomal cysteine protease from the papain superfamily widely expressed in
many mammalian tissues, is the only member of this protease family that consists of four identical subunits. In the acidic lysosomal milieu, CTSC
functions as an amino dipeptidase cleaving depeptides from the N-terminus of its protein substrate. The enzyme is becoming recognized as one of
the most versatile protease-processing system known so far, capable of proteolytic activation of granzymes A, B and C, cathepsin G, neutrophil
elastase and chymase. CTSC-deficient mice fail to activate serine proteases from granules of cytotoxic T-lymphocytes, natural killer cells,
neutrophils, and mast cells. LOF mutations in the gene result in early-onset periodontitis and palmoplantar keratosis, characteristics of Papillon–
Lefevre syndrome (PLS). More than 50 mutations have been identified so far in PLS, all causing loss of CTSC function and activity, which is
associated with subsequent severe reduction in the activity of neutrophil-derived serine proteases. The CTSC activity assay kit is based on
proteolytic hydrolysis of the chromogenic peptide substrate Gly-Phe-p-nitroanilide (Anal Biochem 5:360, 1963). Cleavage of pNA from the
substrate increases absorbance at 405 nm (extinction coefficient= 9.96 mM-1cm-1) and 410 nm (extinction coefficient= 8.8 mM-1cm-1), allowing
for sensitive and quantitative assay of CTSC activity present in tissue/cell lysates. Kit components are stable for at least 1 year if stored and
handled properly.
Kit Components:
10x CTSC Buffer: 0.6 ml, store at 4°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
100x CTSC Substrate: 0.05 ml, store at -20°C (for 100 assays)
100x DTT: 0.1 ml, store at -20°C
MSDS: Sodium acetate, acetic acid, DTT, pNA, DMSO
Related products: AAP Assay, CTSB Assay
Product Description: Cathepsin C (CTSC or DPP-1), an abundant lysosomal cysteine protease from the papain superfamily widely expressed in
many mammalian tissues, is the only member of this protease family that consists of four identical subunits. In the acidic lysosomal milieu, CTSC
functions as an amino dipeptidase cleaving depeptides from the N-terminus of its protein substrate. The enzyme is becoming recognized as one of
the most versatile protease-processing system known so far, capable of proteolytic activation of granzymes A, B and C, cathepsin G, neutrophil
elastase and chymase. CTSC-deficient mice fail to activate serine proteases from granules of cytotoxic T-lymphocytes, natural killer cells,
neutrophils, and mast cells. LOF mutations in the gene result in early-onset periodontitis and palmoplantar keratosis, characteristics of Papillon–
Lefevre syndrome (PLS). More than 50 mutations have been identified so far in PLS, all causing loss of CTSC function and activity, which is
associated with subsequent severe reduction in the activity of neutrophil-derived serine proteases. The CTSC activity assay kit is based on
proteolytic hydrolysis of the chromogenic peptide substrate Gly-Phe-p-nitroanilide (Anal Biochem 5:360, 1963). Cleavage of pNA from the
substrate increases absorbance at 405 nm (extinction coefficient= 9.96 mM-1cm-1) and 410 nm (extinction coefficient= 8.8 mM-1cm-1), allowing
for sensitive and quantitative assay of CTSC activity present in tissue/cell lysates. Kit components are stable for at least 1 year if stored and
handled properly.
Kit Components:
10x CTSC Buffer: 0.6 ml, store at 4°C
10x Cell Lysis Solution: 25 ml, store at 4ºC
100x CTSC Substrate: 0.05 ml, store at -20°C (for 100 assays)
100x DTT: 0.1 ml, store at -20°C
MSDS: Sodium acetate, acetic acid, DTT, pNA, DMSO
Related products: AAP Assay, CTSB Assay
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Biomedical Research Service
& Clinical Application
& Clinical Application
HepG2 cells were treated with
MG132 to induce autophagy. The
CTSC assay shows the induction
of cathepsin C activity during
autophagy.
MG132 to induce autophagy. The
CTSC assay shows the induction
of cathepsin C activity during
autophagy.