ATP/ADP/AMP Assay Kit                                                                                                                                                                                     Price

Product Description: Many metabolic reactions are controlled by the energy status of the cell. One index of the energy status is the energy
charge (EC) of the cell, which is determined by the concentrations of adenine nucleotides. Although EC could theoretically range in values from 0 to
1, most cells maintain a steady-state EC in the range of 0.8 to 0.95, meaning that the energy status of the cell is buffered. ATP, ADP and AMP are
also allosteric effectors for many enzymes. Intracellular adenine nucleotide concentrations vary during cell growth and cell death. Extracellular
adenine nucleotides can regulate various signaling pathways. The assay kit is based on the luciferase reaction in the presence of ATP, which
generates yellow-green light that can be conveniently measured by a luminometer or scintillation counter (Anal. Biochem. 80:496,1977). This
provides the basis for a sensitive ATP assay system capable of detecting 0.1 uM or 1 picomole of ATP. The ATP/ADP/AMP assay kit is sufficient for
200 assays using 0.1 ml of ATP Assay Solution per assay. Sample ADP and AMP are converted to ATP by pyruvate kinase and adenylate kinase
reactions. The ATP Assay Solution if stored in aliquots at -80°C is stable for many years. Repeated freeze-thaw cycles should be avoided.

Kit Components:

ATP Assay Solution: 2 x 10 ml, store at -80°C (for 200 assays)
1 mM ATP Standard: 0.5 ml, store at -80°C
ADP-CB: 0.5 ml, store at -80°C
AMP-CB: 0.5 ml, store at -80°C
EDB: 5 ml, store at -80°C
ADP-CE: 40 ul, store at 4°C (do not freeze)
AMP-CE: 20 ul, store at 4°C (do not freeze)
4 mM EDTA: 10 ml, store at 4°C

MSDS: luciferin, EDTA, Tris, DTT

Related kits: Cell Proliferation Assay, Cell Injury Assay, Phosphate Assay, NAD+ Assay, Pyruvate Assay

Citation:
Wong et al
The Flavone Luteolin Suppresses SREBP-2 Expression and Post-Translational Activation in Hepatic Cells
PLOS ONE 10(8): e0135637. doi:10.1371/journal.pone.0135637, 2015

Yang et al
A pharmacological inhibitor of NLRP3 inflammasome prevents non-alcoholic fatty liver disease in a mouse model induced by high fat diet
Scientific Reports 6:24399, 2016
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