Aldehyde Dehydrogenase (ALDH) Assay Kit                                                                                                                                                 Price

Product Description: In mammals, ethanol is metabolized mainly in the liver by alcohol dehydrogenase (ADH), which oxidizes ethanol to
acetaldehyde. Acetaldehyde, a toxic metabolite responsible for the miserable effects of hangovers, is further oxidized to acetate by aldehyde
dehydrogenase (ALDH). ALDH belongs to a large family of aldehyde dehydrogenases that can be found in many tissues of the body, but are at
the highest concentrations in the liver. ALDH1 and ALDH2 display different abilities to detoxify small aldehydes (such as acetaldehyde) and large
aldehydes (such as retinaldehyde). The assay solution uses acetaldehyde as a substrate, and thus preferentially detects the activity of ALDH2 in
crude extracts. This non-radioactive colorimetric assay is based on the reduction of the tetrazolium salt INT in a NAD
PH-coupled enzymatic
reaction. The INT reaction product is water-soluble and exhibits an absorption maximum at 492 nm. The intensity of the red color formed is
increased in the presence of increased ALDH activity. The enzyme assay solution should be kept at -80ºC, and will remain stable at -80ºC for ~1
year.

Kit Components:

ALDH Assay Solution: 10 ml, store at -80ºC (for 200 wells)
10x ALDH Substrate: 1 ml, store at -80ºC
10x Cell Lysis Solution: 25 ml, store at 4ºC

MSDS: acetaldehyde, TX-100, DMSO, INT, acetic acid, Hepes

Related kits: ADH Enzyme Assay, Ethanol Assay
Site Map
Biomedical Research Service
& Clinical Application
Hamster tissue extracts were prepared
and assayed by ALDH Assay Kit. The
assay shows that the liver exhibits a more
than 30 fold higher ALDH activity than the
heart. Enzyme units per mg proteins are
shown.
Alphabetical Listing