Measuring hexokinase activity in cellular
aging.
Bone marrow mesenchymal stem
cells (MSC) were cultured in vitro. Early and
late passage MSC were harvested for
enzyme assay. The study shows that
hexokinase activity is increased upon
repeated cell passages in vitro.
Welcome to BRSC
- Streamlining Laboratory Medicine -

Biomedical Research Service & Clinical
Application is affiliated with the School of
Medicine and Biomedical Sciences,
University at Buffalo. The center produces
high-quality, cost-effective research and
diagnostic reagents for the broad life
science and clinical communities, and
promotes collaborative translational
research. Please send your inquiry to:

Dr. Techung Lee
Director of BRSC
Department of Biochemistry
& Department of Biomedical Engineering
University at Buffalo
Email: chunglee@buffalo.edu
Tel: (716) 829-3106
Measuring ethanol content in Red Beaujolais
Wine.
The one-step assay shows that the Red
Wine contains ~13% alcohol.
Biomedical Research Service
& Clinical Application
Measuring L-lactate levels in culture
media.
The assay reveals that lactate
production by the Bcl-2 expressor is
reduced compared to the parental
HEK293 cells.
Cell Regulation
O-GlcNAcylation
Cell Viability
Cell Injury
Cell Proliferation
DPP-4 (CD26) Assay
Osteogenesis
Chondrogenesis
Adipogenesis
ECM remodeling
Alkaline Phosphatase Assay
Site Map

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Google Job-http://www.google.com/about/jobs/?gclid=CJ_Iyp7_iLsCFe87MgodSA0AjQ#src=Online/House%20Ads/BKWS&utm_source=adwords&utm_medium=text&utm_campaign=BKWS
Useful websites for biomedical research

Pubmed Tools- http://www.ncbi.nlm.nih.gov/pubmed

Nucleic Acid/Protein Analysis by Colorado State Univ- http://www.vivo.colostate.edu/molkit/index.html

Primer Analysis by Whitehead Institute for Biomedical Research- http://frodo.wi.mit.edu/primer3

Buffer Recipes by Sigma- http://www.sigmaaldrich.com/content/sigma-aldrich/areas-of-interest/life-science/core-bioreagents/biological-buffers/learning-center/buffer-reference-center.html#pH

US National Library of Medicine- http://www.ncbi.nlm.nih.gov/pubmed   

US National Institute of Health- http://www.nih.gov   

NIH Clinical Trials- http://clinicaltrials.gov   

NIH Gene Therapy Program- http://www.gtrp.org                                                                                                                                       

US Patent Office- http://www.uspto.gov

Knockout Mouse Project by UC Davis- http://www.komp.org

Shared Facilities of Roswell Park Cancer Institute- http://www.roswellpark.edu/shared-resources

Shared Facilities of University at Buffalo- http://medicine.buffalo.edu/research/core_facilities.html

American Type Culture Collection- http://www.atcc.org

Sigma MSDS Search- http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do

Histology information by Histonet- http://www.histonet.org
Alphabetical
Listing
Energy Metabolism
Hexokinase (HK)
GAP dehydrogenase (GAPDH)
Lactate dehydrogenase (LDH)
Pyruvate dehydrogenase (PDH)
Malate dehydrogenase (MDH)
Pyruvate Kinase (PK)
Creatine Kinase (CK)
Adenylate Kinase (ADK)
Sorbitol Dehydrogenase (SDH)
L-lactate
D-lactate
ATP/ADP/AMP
Pyruvate
NAD+
Glucose
Glucose-6-phosphate
Amino Acid/Lipid/Nucleotide Metabolism
Glutamate Dehydrogenase (GLDH) Assay
Alanine Aminotransferase (ALT or GPT) Assay
Aspartate Aminotransferase (AST or GOT) Assay
Threonine Dehydrogenase Assay
Inosine-5′-monophosphate Dehydrogenase (IMPDH) Assay
Nucleoside Diphosphate Kinase (NDK) Assay
Glucose-6-phosphate dehydrogenase (G6PD)  
Adenylate Kinase (ADK or myokinase) Assay
Glycerol-3-phosphate dehydrogenase (GPDH) Assay
G6P dehydrogenase (G6PD) Assay
Glutamate Assay
Glutaminase Assay
Tyrosine Aminotransferase (TAT) Assay
GABA Aminotransferase (GABAT) Assay
Detection Systems         
TMB Substrate kit
DAB Substrate kit
BCIP Substrate kit
Alkaline Phosphatase
Horseradish Peroxidase
Luciferase
LacZ   


Proteins/Antibodies


Viral Vectors


cDNA clones
Ethanol Metabolism      
Alcohol dehydrogenase (ADH)
Aldehyde dehydrogenase (ALDH)
Ethanol
Stress Response
Peroxide   
Lipid Peroxidation
Cell Death
Nitric Oxide
Intracellular Thiols
Protease Activation
Catalase Assay
Glutathione Assay
GST Assay
Senescence Detection
Here are some of the
pharmaceutical and biotech
companies we have proudly
served over the years:
L-Lactate Assay
L-Lactate Assay
L-Lactate Assay
Ethanol Assay
Hexokinase Assay
IMPDH Assay
Discovering IMPDH inhibitors. HeLa
cells were treated with DMSO (control)
and 10 uM mycophenolic acid (MPA) for
24 hours, following which cells were
harvested and IMPDH activity was
measured using the IMPDH assay kit.